|
|
|
|
|
| Biller-Takahashi,Jaqueline Dalbello; Takahashi,Leonardo Susumu; Marzocchi-Machado,Cleni Mara; Zanuzzo,Fabio Sabbadin; Sabioni,Rafael Estevan; Urbinati,Elisabeth Criscuolo. |
| The objective of this study was to evaluate the methodology to establish the hemolytic activity of alternative complement pathway as an indicator of the innate immunity in Brazilian fish pacu (Piaractus mesopotamicus), in addition to verifying the influence of β-glucan as an immunostimulant. Fish were fed with diets containing 0, 0.1 and 1% β-glucan, during seven days, and then inoculated with Aeromonas hydrophila. Seven days after the challenge, they were bled for serum extraction. The methodology consisted of a kinetic assay that allows calculating the required time for serum proteins of the complement to promote 50% lysis of a rabbit red blood cell suspension. The method developed in mammals was successfully applied for pacu and determined that the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: A. hydrophila; Β-glucan; Immune response; Pacu. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000200001 |
| |
|
|
| Sabioni,Rafael Estevan; Zanuzzo,Fábio Sabbadin; Cyrino,José Eurico Possebon. |
| Abstract Stress in intensive fish farming hamper immune function of fish and cause losses by disease outbreaks, a situation that can be minimized, but cannot be completely circumvented, by the use of immunomodulators. Addition of immunomodulators to aquafeeds has thus become a common practice. β-glucan (BG) is one of most studied and effective immunomodulators, aquaculture purposes included. Extracted from cell walls of bacteria, fungi and selected cereals, BG activity depends on the source and extraction methods. This study evaluated effects of two BG products (BG1 and BG2), extracted from Saccharomyces cerevisiae under varying extraction methods and with different immune activity, on the feeding of pacu Piaractus mesopotamicus juveniles. BG1 provided... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immunostimulant; Aquaculture; Stress; Aeromonas hydrophila; Leukocytes; Lysozyme. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100410 |
| |
|
|
| Correa,Roselany de Oliveira; Aguilar,Fredy Armando Aguilar; Cruz,Thaline Maira Pachelli da; Sabioni,Rafael Estevan; Cyrino,José Eurico Possebon. |
| ABSTRACT Cost and environmental restrictions limit the use of fishmeal (FM) in aquafeeds and plant protein (PP) sources. Soybean by-products are important alternative; however, their antinutritional factors may reduce fish growth. This study evaluates the effect of FM substitution for PP (soybean meal and soy protein concentrate) in diets of dourado (Salminus brasiliensis), a promising species for fish farming and empirically considered highly tolerant to PP-based diets. A control diet was formulated to contain 20 % of fishmeal and 61 % of plant protein (SBM) and other five diets with decreasing FM graded levels (16 %, 12 %, 8 %, 4 %, 0 %), which were replaced by soybean protein concentrate (SPC), comprising six treatments and four replicates. Fifteen... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteritis; Aquaculture; Protein source; Gastrointestinal tract; Nutrition. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000200400 |
| |
|
|
|
